AQA BiologyCell biology

Cell structure and microscopy

Compare cells and use magnification calculations accurately.

Start here

The key idea

Animal, plant and bacterial cells contain different structures.In exams, link each structure to its function rather than listing names.

Equation to know

magnification = image size / real size

Plant and animal cellsCompare the structures found in each cell type.
Plant and animal cellsCompare the structures found in each cell type.animal cellplant cellnucleuschloroplast
Revision notes

The bit that matters

Learn the process in clean chunks. If a sentence explains a cause, make sure you can say the effect too.

1

Eukaryotic and prokaryotic cells

All cells are either eukaryotic or prokaryotic.Eukaryotic cells (animal and plant cells) have a true nucleus that encloses their DNA.Prokaryotic cells (bacteria) are much smaller and have no nucleus — their DNA floats freely in the cytoplasm as a single loop, sometimes with extra small rings called plasmids.

2

Animal cell structures

Animal cells contain a nucleus (controls the cell and holds DNA), cytoplasm (where reactions happen), a cell membrane (controls what enters and leaves), mitochondria (site of aerobic respiration) and ribosomes (site of protein synthesis).In the exam, always pair each structure with its function.

3

Plant cell structures

Plant cells have everything an animal cell has, plus three extra structures: a cell wall made of cellulose (provides strength and support), chloroplasts (contain chlorophyll and are the site of photosynthesis) and a permanent vacuole (filled with cell sap to keep the cell turgid).

4

Magnification and microscopy

Magnification = image size ÷ real size. Always convert both measurements to the same unit first (1 mm = 1000 µm).Light microscopes magnify up to about 2000x; electron microscopes have far higher magnification and resolution, revealing sub-cellular structures like ribosomes.

Key terms

Definitions to learn

Eukaryotic cell

A cell with a nucleus enclosing its DNA (animal and plant cells).

Prokaryotic cell

A cell with no nucleus; DNA is a single loop in the cytoplasm (bacteria).

Magnification

How many times larger an image is than the real object.

Resolution

The smallest distance between two points that can still be seen as separate.

Organelle

A specialised structure inside a cell with a specific function.

Worked example

A cell image is 24 mm wide. The real cell is 0.06 mm wide. Find the magnification.

1

Convert both measurements to the same unit. 24 mm = 24 mm and 0.06 mm is already in mm, so no conversion needed here.

2

Write the equation: magnification = image size ÷ real size.

3

Substitute: magnification = 24 ÷ 0.06.

4

Calculate: 24 ÷ 0.06 = 400.

Final answer

Magnification = 400x (no units — magnification is a ratio).

Exam habit

Always convert units before applying magnification = image size ÷ real size. Mixing mm and µm gives the wrong answer.

Watch out

Convert units before calculating. Mixing mm and micrometres gives the wrong answer.

Examiner tips

How to score full marks

  • 1Always link each structure to its function — naming the structure alone rarely scores full marks.
  • 2Convert mm and µm to the same unit before any magnification calculation. 1 mm = 1000 µm.
  • 3Magnification has no units — it is a ratio, so never write 'x cm' or 'x mm' after it.
Quick-fire quiz

Test yourself

Pick an answer — you'll see instantly if it's right.

Which structure is found in plant cells but NOT animal cells?

Magnification = 500×. Image size = 5 mm. What is the real size?

Which organelle is the site of aerobic respiration?

Why do electron microscopes reveal more detail than light microscopes?

What does a prokaryotic cell lack that a eukaryotic cell has?

Practice questions

Try these yourself

Open each answer only after you have explained the full biological process.

1Name two structures found in plant cells but not animal cells.[2 marks]
Mark scheme
  1. 1.Recall the structures that support photosynthesis and rigidity.
Any two from: cell wall (1), chloroplasts (1), permanent vacuole (1).
2State one function of the cell membrane.[1 mark]
Mark scheme
  1. 1.Think about what enters and leaves the cell.
Controls what substances enter and leave the cell (1).
3Describe the difference between a eukaryotic and a prokaryotic cell.[2 marks]
Mark scheme
  1. 1.Compare where the genetic material is found.
Eukaryotic cells have a nucleus enclosing the DNA (1); prokaryotic cells have no nucleus — the DNA is a single loop free in the cytoplasm, plus possible plasmids (1).
4Explain why sperm cells contain many mitochondria.[2 marks]
Mark scheme
  1. 1.Mitochondria release energy by aerobic respiration.
  2. 2.Link the energy to the cell's job.
Mitochondria release energy by aerobic respiration (1); sperm need this energy to swim to the egg (1).
5An image is 15 mm long at 300x magnification. Find the real length.[2 marks]
Mark scheme
  1. 1.Rearrange magnification = image ÷ real to make real the subject.
  2. 2.Substitute and divide.
real size = image size ÷ magnification = 15 ÷ 300 = 0.05 mm (1). Convert if asked: 0.05 mm = 50 µm (1).
6A bacterial cell is 2 µm long. An image of it is 8 mm long. Calculate the magnification.[2 marks]
Mark scheme
  1. 1.Convert both to the same unit first.
  2. 2.8 mm = 8000 µm.
  3. 3.Use magnification = image ÷ real.
Convert 8 mm to 8000 µm (1); magnification = 8000 ÷ 2 = 4000x (1).
7Describe how to use a light microscope to observe onion cells.[3 marks]
Mark scheme
  1. 1.Order the steps from slide preparation to focusing.
Prepare a slide with the specimen and a cover slip (1); start with the lowest-power objective lens (1); use the coarse focus to bring the cells into view, then the fine focus for a sharp image (1).
8Explain why an electron microscope allows scientists to see sub-cellular structures that a light microscope cannot.[2 marks]
Mark scheme
  1. 1.Compare resolution and magnification of the two instruments.
An electron microscope has a much higher magnification and higher resolution (resolving power) (1); this allows smaller structures such as ribosomes and internal detail of mitochondria to be seen clearly (1).
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